Gallery HS-AFM Images + Videos
This website is dedicated to beta-testers of our Ultra-Short Cantilevers and their research as well as to all groups working on High Speed Scanning worldwide. It should serve as a platform where everyone who is interested can show and observe what is possible with High Speed AFM (HS-AFM).
You are all cordially invited to participate in this site. If you have interesting images, videos, papers to share with others please feel free to contact: info@highspeedscanning.com.
If you would like to participate in the beta-testing of our probes for high-speed scanning please contact: info@highspeedscanning.com.
Images:
High resolution image of bacteriorhodopsin taken in dynamic mode with an oscillation amplitude of 0.8 nm. The scan size was 127 nm x 62 nm (495 x 200 pixels) and the line rate was 7 Hz. The cantilever used for this application was a USC-NM-2MHz (10 μm long, 5 μm wide and 280 nm thick) which has a Resonance Frequency of 2 MHz in air (1.1 MHz in buffer solution) and a Force Constant of 3 N/m.
High-speed contact mode image of collagen fibrils in air with a line rate of 210 Hz and scan size of 21 µm × 21 μm. A small silicon nitride cantilever with a length of 20 µm, a width of 2.5 µm, and a resonant frequency of 2.2 MHz was used. Its tip moved with a velocity of 8.8 mm/s relative to the sample surface. (a) 3D-rendered topography. The image was recorded with a very high resolution of 2000 × 2000 pixels. (b) Off-line zoom-in into the framed area in (a). The corresponding deflection instead of the topography is shown here. The 67 nm bending pattern of the collagen fibrils can be clearly identified (arrow).
High-speed image sequence of calcite dissolution in diluted hydrochloric acid at a rate of 10 images/s with a small silicon cantilever (length 18 µm, width 4.5 µm, resonant frequency 1.1 MHz in air and 440 kHz in water, spring constant 1.0 N/m). Four topography images of a sequence of 20 are shown. 100 lines of 400 pixels each were recorded for each image, corresponding to a line rate of 1.0 kHz. Calcite dissolution occurs mainly at the edges of the 3 Å high molecular layers. The edges of the upper calcite layer in frame 1 are marked by dashed lines in the other frames. Scan size: 1 µm × 1 µm.
Contact mode image of the muscovite mica {001} cleavage plane in water. A small silicon nitride cantilever with a length of 20 µm, a width of 7.5 µm, a resonant frequency of 641 kHz in air and 322 kHz in water, and a spring constant of 0.7 N/m was used. The image was taken with a scan size of 2.8 nm × 2.8 nm and a line rate of 63 Hz. The contrast in the height image is enhanced by atomic stick-slip friction. The hexagonal lattice structure of the cleavage plane can be clearly identified (a ≈ 5.2 Å, b ≈ 9.0 Å). The arrangement of the SiO4 and AlO4- tetrahedrons is indicated by small white triangles.
a) FM topography image of a freshly cleaved mica in solution (150 mM KCl and 20 mM Tris-HCl in 18.2 MΩ DI water). The image was acquired with a NanoWorld Arrow™ UHF cantilever (f0 = 511.9 kHz, setpoint = +1650 Hz, A = 1.58 nm, tip speed = 160 nm/s, scan size = 3.5 nm x 3.5 nm, Z-scale = 83 pm)
b) Ball-and-stick model of the mica surface
Videos:
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CELGARD
Properties: Mode: Non-Contact Mode Line Rate (LR): 10 Hz Scan Size (S): 1 μm x 1 μm Resolution (R): 400 x 400 pixels Tip Speed (TS): 0.02 mm/s Probe: Arrow™ UHF 1.2 MHz Video by AIST-NT (www.aist-nt.com) on a SmartSPM-1000 » PLAY VIDEO |
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CELGARD
Properties: Mode: Non-Contact Mode Line Rate (LR): 15 Hz Scan Size (S): 1 μm x 1 μm Resolution (R): 400 x 400 pixels Tip Speed (TS): 0.03 mm/s Probe: Arrow™ UHF 1.2 MHz Video by AIST-NT (www.aist-nt.com) on a SmartSPM-1000 » PLAY VIDEO |
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HOPG
Properties: Mode: Non-Contact Mode Line Rate (LR): variable (from 10 Hz to 60 Hz) Scan Size (S): 8 μm x 8 μm Resolution (R): 512 x 512 pixels Tip Speed (TS): variable (from 0.16 mm/s to 0.96 mm/s) Probe: Arrow™ UHF 1.1 MHz Video by AIST-NT (www.aist-nt.com) on a SmartSPM-1000 » PLAY VIDEO |
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HOPG
Properties: Mode: Non-Contact Mode Line Rate (LR): 30 Hz Scan Size (S): 8 μm x 8 μm Resolution (R): 512 x 512 pixels Tip Speed (TS): 0.48 mm/s Probe: Arrow™ UHF 1.1 MHz Video by AIST-NT (www.aist-nt.com) on a SmartSPM-1000 » PLAY VIDEO |
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HOPG
Properties: Mode: Non-Contact Mode Line Rate (LR): 50 Hz Scan Size (S): 8 μm x 8 μm Resolution (R): 512 x 512 pixels Tip Speed (TS): 0.80 mm/s Probe: Arrow™ UHF 1.1 MHz Video by AIST-NT (www.aist-nt.com) on a SmartSPM-1000 » PLAY VIDEO |
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HOPG
Properties: Mode: Non-Contact Mode Line Rate (LR): 70 Hz Scan Size (S): 8 μm x 8 μm Resolution (R): 512 x 512 pixels Tip Speed (TS): 1.12 mm/s Probe: Arrow™ UHF 1.1 MHz Video by AIST-NT (www.aist-nt.com) on a SmartSPM-1000 » PLAY VIDEO |
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DVD topography
Properties: Mode: Contact Mode Line Rate (LR): 796 Hz Image Rate (IR): 6.86 Hz Scan Size (S): variable (from 1.6 μm x 1.6 μm to 6.8 μm x 6.8 μm) Resolution (R): 100 x 100 pixels Tip Speed (TS): variable (from 2.54 mm/s to 10.82 mm/s) Probe: USC-NM 2 MHz Video by C. Braunsmann and T. E. Schäffer, University of Erlangen-Nuremberg, Germany » PLAY VIDEO |
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DVD topography
Properties: Mode: Contact Mode Line Rate (LR): 1243 Hz Image Rate (IR): 8.4 Hz Scan Size (S): variable (from 0.78 μm x 0.78 μm to 5.2 μm x 5.2 μm) Resolution (R): 128 x 128 pixels Tip Speed (TS): variable (from 1.94 mm/s to 12.92 mm/s) Probe: USC-NM 2 MHz Video by C. Braunsmann and T. E. Schäffer, University of Erlangen-Nuremberg, Germany » PLAY VIDEO |
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DVD topography
Properties: Mode: Contact Mode Line Rate (LR): 1592 Hz Image Rate (IR): 14 Hz Scan Size (S): variable (from 1.15 μm x 1.15 μm to 6 μm x 6 μm) Resolution (R): 100 x 100 pixels Tip Speed (TS): variable (from 3.66 mm/s to 19.1 mm/s) Probe: USC-NM 2 MHz Video by C. Braunsmann and T. E. Schäffer, University of Erlangen-Nuremberg, Germany » PLAY VIDEO |
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Dissolution of a calcite crystal in diluted HCl
Properties: Mode: Contact Mode Line Rate (LR): 1000 Hz Image Rate (IR): 10 Hz Scan Size (S): 1 μm x 1 μm Resolution (R): 100 x 400 pixels Tip Speed (TS): 2 mm/s Probe: USC-Si 1.1 MHz Video by C. Braunsmann and T. E. Schäffer, University of Erlangen-Nuremberg, Germany » PLAY VIDEO |
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Dissolution of a calcite crystal in diluted HCl
Properties: Mode: Contact Mode Line Rate (LR): 1300 Hz Image Rate (IR): 13 Hz Scan Size (S): 1 μm x 1 μm Resolution (R): 100 x 400 pixels Tip Speed (TS): 1 mm/s Probe: USC-Si 1.1 MHz Video by C. Braunsmann and T. E. Schäffer, University of Erlangen-Nuremberg, Germany » PLAY VIDEO |