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Gallery: HS-AFM Videos

Ice crystallization on mica
Temperature variation from +1° to -10°C
Properties:
Line Rate (LR): 80 Hz
Image Rate (IR): 0.4 Hz
Scan Size (S): 5 µm x 5 µm
Resolution (R): 200 x 200 pixels
Tip Speed (TS): 0.80 mm/s
Probe: NanoWorld Arrow™ UHF

Video by Keysight Technologies (www.keysight.com) with Quick Scan on a 9500 Atomic Force Microscope (AFM)

» PLAY VIDEO
Live cell (COS7)  - Video 2
Properties:
Mode: Phase Modulation Mode
Line Rate (LR): 60 Hz
Image Rate (IR): 0.2 Hz
Scan Size (S): 4 µm x 3 µm
Resolution (R): 320 x 240 pixels
Tip Speed (TS): 0.48 mm/s
Probe: USC-F0.8-k0.1 (customized)

Video by A. Yagi, N. Sakai, Y. Uekusa, S. Ito, Olympus Corp., Tokyo, Japan
» PLAY VIDEO
Live cell (COS7)  - Video 1
Properties:
Mode: Phase Modulation Mode
Line Rate (LR): 60 Hz
Image Rate (IR): 0.2 Hz
Scan Size (S): 4 µm x 3 µm
Resolution (R): 320 x 240 pixels
Tip Speed (TS): 0.48 mm/s
Probe: USC-F0.8-k0.1 (customized)

Video by A. Yagi, N. Sakai, Y. Uekusa, S. Ito, Olympus Corp., Tokyo, Japan
» PLAY VIDEO
Grating with 22 nm Step Height
Properties:
Mode: Dynamic Mode (Non-Contact) 
Line rate (LR): variable (from 50 Hz to 110 Hz)
Image rate (IR): variable (from 0.4 Hz to 0.85 Hz)
Scan Size (S): 5 µm x 5 µm
Resolution (R): 128 x 128 pixels
Tip Speed (TS): variable (from 0.50 mm/s to 1.10 mm/s)
Probe: NanoWorld Arrow™ UHF

Video by Anfatec Instruments AG (www.anfatec.de) on a Anfatec Level-AFM with AFT-HSC50 controller 
» PLAY VIDEO
Streptavidin crystal on a biotin containing lipid bilayer
Properties:
Mode: Tapping Mode
Line Rate (LR): 600 Hz   
Image Rate (IR): 1 Hz
Scan Size (S): 0.03 μm x 0.03 μm
Resolution (R): 120 x 120 pixels
Tip Speed (TS): 0.04 mm/s
Probe: NanoWorld USC-F1.2-k0.15

Video by A. Karner and J. Preiner, Center for Advanced Bioanalysis (CBL), Linz, Austria
» PLAY VIDEO
Exocytic event and Cytoskeleton movement in COS7 live cell
Properties:
Mode: Phase Modulation Mode
Line Rate (LR): 18 Hz
Image Rate (IR): 0.1 Hz
Scan Size (S): 4 μm x 3 μm
Resolution (R): 192 x 144 pixels
Tip Speed (TS): 0.14 mm/s
Probe: USC-F0.8-k0.1 (customized)

Video by A. Yagi, N. Sakai, Y. Uekusa, S. Ito, Olympus Corp., Tokyo, Japan
» PLAY VIDEO
Live cell (COS7) Mitochondria-GFP
Properties:
Mode: Phase Modulation Mode
Line Rate (LR): 18 Hz
Image Rate (IR): 0.1 Hz
Scan Size (S): 4 μm x 3 μm
Resolution (R): 192 x 144 pixels
Tip Speed (TS): 0.14 mm/s
Probe: USC-F0.8-k0.1 (customized)

Video by A. Yagi, N. Sakai, Y. Uekusa, S. Ito, Olympus Corp., Tokyo, Japan
» PLAY VIDEO
DNA Origami in buffer
Properties:
Mode: Amplitude Modulation Mode in buffer
Line Rate (LR): 105 Hz
Image Rate (IR): 0.41 Hz
Scan Size (S): 0.05 μm x 0.05 μm
Resolution (R): 256 x 256 pixels
Tip Speed (TS): 0.01 mm/s
Probe: NanoWorld USC-F0.3-k0.3

Video by JPK Instruments (www.jpk.com) on a NanoWizard ULTRA Speed AFM
Sample courtesy of Dr. Rebecca Meyer, Prof. Christof M. Niemeyer, Karlsruhe Institute of Technology (KIT), Germany
» PLAY VIDEO
Polyhydroxybutyrate-co-valerate (PHB/V) spherulite crystallization growth front
Properties:
Mode: Amplitude Modulation Mode
Line Rate (LR): 60 Hz
Image Rate (IR): 0.24 Hz (played at 5.6 Hz)
Scan Size (S): 1.5 μm x 1.5 μm
Resolution (R): 512 x 256 pixels
Tip Speed (TS): 0.18 mm/s
Probe: SD-USC-Si-2.2MHz

Video by JPK Instruments (www.jpk.com) on a NanoWizard ULTRA Speed AFM
Sample courtesy of Prof. Jamie Hobbs, University of Sheffield, UK
» PLAY VIDEO
Single-Molecules Imaging of micro-Brownian Movement in a Chiral Helical (pi)-Conjugated Polymer
Properties:
Mode: Dynamic Mode
Line Rate (LR): 240 Hz
Image Rate (IR): 2.0 Hz (played at 30 Hz)
Scan Size (S): 0.4 μm x 0.3 μm
Resolution (R): 128 x 96 pixels
Tip Speed (TS): 0.19 mm/s
Probe: NanoWorld USC-F1.2-k0.15

Video by M. Grewal and K. Shinohara, JAIST, Japan
» PLAY VIDEO
Calcite crystal growth
Properties:
Mode: Phase Modulation Mode
Line Rate (LR): 100 Hz
Image Rate (IR): 1 Hz
Scan Size (S): 0.01 μm x 0.005 μm
Resolution (R): 200 x 100 pixels
Tip Speed (TS): 0.002 mm/s
Probe: NanoWorld USC-F5-k30

Video by K. Miyata, H. Asakawa and T. Fukuma, Kanazawa University, Japan. Applied Physics Letters 103 (2013) 203104
» PLAY VIDEO
Lamellipodium on COS7 with USC movement
Properties:
Mode: Phase Modulation Mode
Line Rate (LR): 18 Hz
Image Rate (IR): 0.1 Hz
Scan Size (S): 4 μm x 3 μm
Resolution (R): 192 x 144 pixels
Tip Speed (TS): 0.14 mm/s
Probe: NanoWorld USC-F1.2-k0.15

Video by A. Yagi, N. Sakai, Y. Uekusa, S. Ito, Olympus Corp., Tokyo, Japan
» PLAY VIDEO
Lamellipodium on COS7
Properties:
Mode: Phase Modulation Mode
Line Rate (LR): 18 Hz
Image Rate (IR): 0.1 Hz
Scan Size (S): 4 μm x 3 μm
Resolution (R): 192 x 144 pixels
Tip Speed (TS): 0.14 mm/s
Probe: NanoWorld USC-F1.2-k0.15

Video by A. Yagi, N. Sakai, Y. Uekusa, S. Ito, Olympus Corp., Tokyo, Japan
» PLAY VIDEO
Lamellipodium on  stained COS7
Properties:
Mode: Phase Modulation Mode
Line Rate (LR): 18 Hz
Image Rate (IR): 0.1 Hz
Scan Size (S): 4 μm x 3 μm
Resolution (R): 192 x 144 pixels
Tip Speed (TS): 0.14 mm/s
Probe: NanoWorld USC-F1.2-k0.15

Video by A. Yagi, N. Sakai, Y. Uekusa, S. Ito, Olympus Corp., Tokyo, Japan
» PLAY VIDEO
Lamellipodium on non stained COS7
Properties:
Mode: Phase Modulation Mode
Line Rate (LR): 18 Hz
Image Rate (IR): 0.1 Hz
Scan Size (S): 4 μm x 3 μm
Resolution (R): 192 x 144 pixels
Tip Speed (TS): 0.14 mm/s
Probe: NanoWorld USC-F1.2-k0.15

Video by A. Yagi, N. Sakai, Y. Uekusa, S. Ito, Olympus Corp., Tokyo, Japan
» PLAY VIDEO
OmpF trimers
Properties:
Mode: Non-Contact Mode
Line Rate (LR): 1258 Hz
Image Rate (IR): 2.09 Hz
Scan Size (S): 0.075 μm x 0.075 μm
Resolution (R): 300 x 300 pixels
Tip Speed (TS): 0.19 mm/s
Probe: NanoWorld USC-F1.5-k0.6

Video by I. Casuso and S. Scheuring, U1006 INSERM, Aix-Marseille University, France
» PLAY VIDEO
CELGARD
Properties:
Mode: Non-Contact Mode
Line Rate (LR): 10 Hz
Scan Size (S): 1 μm x 1 μm
Resolution (R): 400 x 400 pixels
Tip Speed (TS): 0.02 mm/s
Probe: NanoWorld Arrow™ UHF

Video by AIST-NT (www.aist-nt.com) on a SmartSPM-1000
» PLAY VIDEO
CELGARD
Properties:
Mode: Non-Contact Mode
Line Rate (LR): 15 Hz
Scan Size (S): 1 μm x 1 μm
Resolution (R): 400 x 400 pixels
Tip Speed (TS): 0.03 mm/s
Probe: NanoWorld Arrow™ UHF

Video by AIST-NT (www.aist-nt.com) on a SmartSPM-1000
» PLAY VIDEO
HOPG
Properties:
Mode: Non-Contact Mode
Line Rate (LR): variable (from 10 Hz to 60 Hz)
Scan Size (S): 8 μm x 8 μm
Resolution (R): 512 x 512 pixels
Tip Speed (TS): variable (from 0.16 mm/s to 0.96 mm/s)
Probe: NanoWorld Arrow™ UHF

Video by AIST-NT (www.aist-nt.com) on a SmartSPM-1000
» PLAY VIDEO
HOPG
Properties:
Mode: Non-Contact Mode
Line Rate (LR): 30 Hz
Scan Size (S): 8 μm x 8 μm
Resolution (R): 512 x 512 pixels
Tip Speed (TS): 0.48 mm/s
Probe: NanoWorld Arrow™ UHF

Video by AIST-NT (www.aist-nt.com) on a SmartSPM-1000
» PLAY VIDEO
HOPG
Properties:
Mode: Non-Contact Mode
Line Rate (LR): 50 Hz
Scan Size (S): 8 μm x 8 μm
Resolution (R): 512 x 512 pixels
Tip Speed (TS): 0.80 mm/s
Probe: NanoWorld Arrow™ UHF

Video by AIST-NT (www.aist-nt.com) on a SmartSPM-1000
» PLAY VIDEO
HOPG
Properties:
Mode: Non-Contact Mode
Line Rate (LR): 70 Hz
Scan Size (S): 8 μm x 8 μm
Resolution (R): 512 x 512 pixels
Tip Speed (TS): 1.12 mm/s
Probe: NanoWorld Arrow™ UHF

Video by AIST-NT (www.aist-nt.com) on a SmartSPM-1000
» PLAY VIDEO
DVD topography
Properties:
Mode: Contact Mode
Line Rate (LR): 796 Hz
Image Rate (IR): 6.86 Hz
Scan Size (S): variable (from 1.6 μm x 1.6 μm to 6.8 μm x 6.8 μm)
Resolution (R): 100 x 100 pixels
Tip Speed (TS): variable (from 2.55 mm/s to 10.83 mm/s)
Probe: NanoWorld USC-F2-k3

Video by C. Braunsmann and T. E. Schäffer, University of Erlangen-Nuremberg, German
» PLAY VIDEO
DVD topography
Properties:
Mode: Contact Mode
Line Rate (LR): 1243 Hz
Image Rate (IR): 8.4 Hz
Scan Size (S): variable (from 0.78 μm x 0.78 μm to 5.2 μm x 5.2 μm)
Resolution (R): 128 x 128 pixels
Tip Speed (TS): variable (from 1.94 mm/s to 12.93 mm/s)
Probe: NanoWorld USC-F2-k3

Video by C. Braunsmann and T. E. Schäffer, University of Erlangen-Nuremberg, Germany
» PLAY VIDEO
DVD topography
Properties:
Mode: Contact Mode
Line Rate (LR): 1592 Hz
Image Rate (IR): 14 Hz
Scan Size (S): variable (from 1.15 μm x 1.15 μm to 6 μm x 6 μm)
Resolution (R): 100 x 100 pixels
Tip Speed (TS): variable (from 3.66 mm/s to 19.10 mm/s)
Probe: NanoWorld USC-F2-k3

Video by C. Braunsmann and T. E. Schäffer, University of Erlangen-Nuremberg, Germany
» PLAY VIDEO
Dissolution of a calcite crystal in diluted HCl
Properties:
Mode: Contact Mode
Line Rate (LR): 1000 Hz
Image Rate (IR): 10 Hz
Scan Size (S): 1 μm x 1 μm
Resolution (R): 100 x 400 pixels
Tip Speed (TS): 2.00 mm/s
Probe: SD-USC-Si-1.1MHz

Video by C. Braunsmann and T. E. Schäffer, University of Erlangen-Nuremberg, Germany
» PLAY VIDEO
Dissolution of a calcite crystal in diluted HCl
Properties:
Mode: Contact Mode
Line Rate (LR): 1300 Hz
Image Rate (IR): 13 Hz
Scan Size (S): 1 μm x 1 μm
Resolution (R): 100 x 400 pixels
Tip Speed (TS): 2.60 mm/s
Probe: SD-USC-Si-1.1MHz

Video by C. Braunsmann and T. E. Schäffer, University of Erlangen-Nuremberg, Germany
» PLAY VIDEO